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Each formulation dilution was assessed in triplicate.
Each cell line was assessed in triplicate.
The stability of 111In-DTPA-11B6 was assessed in triplicate by incubating the compound at 4°C in PBS buffer or at 37°C in murine serum collected from normal NMRI mice.
Each sample was assessed in triplicate and analyzed in 3 independent experiments.
Each sample was assessed in triplicate and each experiment was performed at least 3 times.
After centrifugation (20,000 g, 15 min), the absorption of the supernatant at 405 nm was assessed in triplicate in a clear 96 well plate with a Versamax microplate reader (Molecular Devices).
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The recovery of Leptospira DNA was assessed in triplicates by the quantitative PCR (qPCR) assay, described below.
Then, neuronal survival was assessed in triplicates by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay.
For ELISA, 50 µL of supernatant were assessed in triplicate in 96-well ELISA microplates (Greiner Bio One).
Each experiment was run twice with a least 6 animals per group, and in both cases, samples were assessed in triplicate.
Each reaction was run twice with a least 6 animals per group, and in both cases, samples were assessed in triplicate.
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