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Each library sequence set (Table 1) was assembled separately to obtain specific UniGene assemblies for every gene library and every pine species (Table 2); P. pinaster comprehensive assembly was also performed with MIRA3 with mixed 454-Sanger settings.
Each run was assembled separately with the de novo short-read assembler Velvet v0.7.48 [23], using empirically optimized parameters, and the two assemblies subsequently combined using the minimus2 script from AMOS v2.0.8 [75] The contigs were oriented and ordered according to the finished reference genome for ATCC 13124 using Mummer v3.21 [24] and custom perl scripts.
When multiple probes were utilized, each probe was assembled separately and then mixed in SLO/medium just prior to delivery in equimolar concentrations.
Each developmental stage was assembled separately and shared transcripts were collapsed using Cuffmerge [ 23].
The 37121 bp mitochondrion genome was assembled separately in a single contig with AllPathsLG.
Each of the three Illumina read sets for each of the three genotypes was assembled separately.
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In order to take platform dependent sequencing artifacts into consideration, 454 and Illumina reads were assembled separately using different assembly programs.
These two data sets were assembled separately using the EST sequence assembler geneDistiller Scheibye-Alsing KK et al: EST assembly with genedistiller, submitted), resulting in, respectively, 420 and 1,207 contigs and 1,205 and 4,438 singlets.
These two data sets were assembled separately using the EST sequence assembler geneDistiller Scheibye-Alsing KK et al: EST assembly with genedistiller, submitted), and the resulting Heliconius contigs and singlets are available in additional files 4 and 5 respectively.
The reads from each library were assembled separately by Roche Newbler (GS-assembler) version 2.3.
Using MIA Mapping Iterative Assemblerr available at https://github.com/udo-stenzel/mapping-iterative-assembler, last accessed December 2013) mitochondrial genomes were assembled separately for each library.
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