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Standard β-mannanase activity was assayed via the 3,5-dinitrosalicylic acid (DNS) method (Miller 1959).
The amount of residual sulfur in the oxide phosphors calcined at 1100 °C was assayed via ICP elemental analysis to be up to 0.18 wt.% in our previous work [50].
The thus-generated DMT cation in the supernatant was assayed via UV vis spectroscopy, and the loading achieved was found to be 13.2 ± 0.3 nmol mg−1 of pSi particles, comparable with the loading levels of conventional CPG (15.0 nmol mg−1 of resin).
IFNγ secretion from these CD8+ T cells was assayed via intracellular cytokine staining (ICS).
Serum insulin was assayed via radio-immunoassay (Linco Research, St Charles, Missouri, USA).
Expression was assayed via dot blotting and SDS-PAGE western blotting using the mouse monoclonal antibody rho1D4.
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Morphogenesis and growth kinetics were assayed via transmission electron microscopy and viral titering, respectively.
Cellular proliferation and the mesangial matrix in glomeruli were assayed via histological and morphometric procedures.
Bait prey interactions are assayed via the redistribution of the fluorescent prey.
Samples were assayed via polyacrylamide gel electrophoresis (SDS-PAGE) under both reducing and denaturing conditions.
After double ether steroid extraction (85% recovery), androgens (5α-DHT and T) were assayed via radioimmunassay (RIA; 35) for chicks from both species.
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