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Catalase activity was assay of hydrogen peroxide based on the formation of its stable complex with ammonium molkbdate and the OD was measured at 405 nm (Fang et al. [2004]).
Immunoreactivity of at least one antibody was assay dependent as evidenced by Fab 4 and recognition of STEBVax, but not wild-type SEB, in an ELISA.
Lipase activity in the porcine pancreas was assay as described previously [ 19].
The CCs culture medium was collected and stored at −20°C until the progesterone or estradiol was assay.
The loading buffer was assay buffer supplemented with 2 μM Fluo-4 AM (dissolved in 50 50 DMSO/pluronic acid, Invitrogen, UK).
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Each sample was assayed in triplicate.
Proliferation was assayed by bromodeoxyuridine incorporation.
Plant-derived rAChE activity was assayed as previously described10.
HA protease was assayed by its hemagglutination and protease activity.
The enhanced slowing response was assayed as described6.
Luciferase activity was assayed 48 h post-transfection.
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