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Succinate confirmation was ascertained utilizing 200 µL of sample, 60 µg/mL of membrane fraction collected from P. fluorescens, and 2.5 mg/mL of DCIP (dichloroindophenol) in equilibration buffer.
The number of viable cells was ascertained utilizing a MTT (3- 4, 5-dimehylthiazol-2-yl -2-5-dyphennyltetrazolium bromide) assay after 48 hrs.
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Change in free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) for the adsorption procedure were ascertained utilizing the Eqs.
Mortality was ascertained through verbal autopsies.
Mortality was ascertained in 2007.
Muscle mass was ascertained from DXA scanning.
The purity was ascertained by SDS-PAGE.
Glucocorticoid exposure was ascertained from dispensary data.
Blood pressure was ascertained under standardized conditions.
Use of animal models to study OAB can be problematic because bladder symptoms cannot be ascertained in an animal and surrogate physiologic and behavioral outcomes have to be utilized.
Additionally, the chemical mechanism utilized by the metal-binding motif for endonucleolytic activity remains to be ascertained.
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