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Pairwise linkage disequilibrium (LD) was ascertained using the Linkage Disequilibrium Analyzer 1.0 software (Ding et al. 2003).
The system ability to perform titrations was ascertained using the precipitation reaction of vitamin B6 with tetraphenylborate.
The presence of antepartum depression was ascertained using the 9 item Spanish-language Patient Health Questionnaire-9 (PHQ-9) thas has been validated in this population [31 33].
Additional drug use and addiction and psychiatric symptom severity was ascertained using the ASI version 5 (McLellan et al. [1992]) as well as psychiatric symptom severity for the 30 days prior to incarceration.
Depressive symptoms were ascertained using the Centre for Epidemiological Studies - Depression Scale (CES-D), while exercise self-efficacy was ascertained using the Stanford Self-Efficacy scale; both measures have been validated [40,41].
For each of the 500 repetitions, classification accuracy was ascertained using the test data.
The quality of the mouse RNA was ascertained using the Agilent Bioanalyser 2100 using the NanoChip protocol.
The activity in experimental samples was ascertained using the standard curve of cpm versus the dilution of recombinant IL-2 in the supernatant.
The activity in experimental samples was ascertained using the standard curve of mean absorbance values (OD) versus the dilution of recombinant IFN-γ in the supernatant.
Anxiety was ascertained using the anxiety subscale of the Hospital Anxiety and Depression Scale (HADS)[17]; a total score lower than 8, 8 to 10, and greater or equal 11 indicating no, questionable or significant anxiety, respectively.
For those who could not provide written documentation of HIV test results within the past 6 months, HIV serostatus was ascertained using the Determine HIV ½ lateral flow qualitative rapid immunoassay on whole blood (Inverness Medical Innovations Inc).
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