The integrity of the DNA samples was ascertained by positive amplification of human DNA ß-globin control.
Biopsies confirmation was carried out whenever possible and the proof of invasion of central nervous system (CNS) was ascertained by positive cytologic findings in cerebrospinal fluid.
Specificity was ascertained by treating positive control sections as described above but omitting the primary antibodies.
For this study, a combined child-parent diagnosis was ascertained where a positive diagnosis was reported for any one of the three depressive disorders when self- and parent-reported criteria endorsements were combined, such that if a criterion received a positive endorsement by either child or parent, it was considered to be present.
A participant's treatment status for mental health problems was ascertained by a positive answer to the question, "Are you now taking medicine or receiving treatment from a doctor or other health professional for any type of mental health condition or emotional problem?" Participants' diabetes status was determined by self-report.
Secondly, there was no participant was ascertained with smear-positive, recurrent PTB, or multi-drug resistant PTB in this study.
In European countries, a large proportion of 3GC-resistant K. pneumoniae isolates, ranging between 85 % and 100%%, was ascertained as ESBL-positive [ 1].
According to the routine clinical guideline, TB was ascertained either by a positive sputum smear, suggestive chest X-ray, suggestive pathology or a positive sputum culture [ 23].
The concordance between admission type in the DNPR and in the medical records was ascertained with estimates of the positive predictive value (PPV), sensitivity, and specificity with corresponding 95% confidence intervals (CI).
Approximate timing of infection for patients with IgG-positive samples was ascertained by using a commercial solid-phase enzyme immunoassay (Toxoplasma gondii IgG Avidity EIA; Ani Labsystems Ltd. Oy, Vantaa, Finland) to determine avidity (binding ability) index.
