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The response was arbitrarily quantified with scores from one to seven, where one represented a bee that only responded to one concentration of sucrose (usually the 50% w/w), while a score of seven represented an individual that responded to all concentrations tested.
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To ensure that the PCR products from each sample were comparable the PCRs were halted during the exponential amplification phase and product signal intensities were arbitrarily quantified using densitometry and normalised against the 18S endogenous control.
The cyclin D1 or E band was quantified by densitometry, and the band intensities normalised to the strongest signal, which was arbitrarily defined as 100.
The list was arbitrarily capped at eight.
Each control was arbitrarily set at 100%.
ratio of 0.5 was arbitrarily chosen, ΔlogL/S.E.
The threshold of 15% was arbitrarily chosen.
The similarity value 0.30 was arbitrarily chosen.
In order to confirm the results of the Illumina sequencing and quantify the expression patterns of both the miRNA and their target transcripts, nine miRNA-target gene pairs were arbitrarily selected for further analyses by qRT-PCR.
They are arbitrarily selected and easily dismissed.
Deficits are arbitrarily defined and easily manipulated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com