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In OV2008 cells, a constitutively active AAkt2 plasmid was applied to activate PI3K/Akt signaling.
HEK293T cells were mock transfected or transfected PLpro-TM with HA-tagged STING (A), Flag-tagged TBK1 (B), or Flag-tagged IKKε (C) which was applied to activate IRF3 pathway.
To study the ion movements underlying the conductance, a voltage step to +57 mV was applied to activate the current, followed by steps back to between −33 and +17 mV.
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Results showed that the ambient temperature can be applied to activate mixes with 100% POFA.
The concept of multimode switching is applied to activate either a 'bang bang' controller or an adaptive neuro-fuzzy model reference controller (ANFMRC).
The plasmids expressing HA-STING (A and B), Flag-TBK1 (C and D), or Flag-IKKε (E and F) were applied to activate the IFN expression pathway and IRF3-dependent IFN expression pathway, in the presence or absence of PLpro-TM-V5 PLpro-TM-V5 PLpro-TM-V5
Radiation treatment of modest dose (10 kGy) has been applied to activate a reactive additive (epoxy-acrylate, 2% to the PET) to bond together the matrix (recycled PET) and the reinforcement (chopped glass fiber, 10 20% to the PET).
The MLTC-based protocol has been applied to activate and expand in vitro circulating T cells from cutaneous melanoma patients.
After centrifugation the lysate (about 500 μg) was applied to the activated amino link resin beads.
Before CaP coating, a plasma surface treatment was applied to clean and activate the PCL surface for calcium and phosphate ion grafting.
Hydrogen peroxide paste was applied to five samples and activated using a halogen lamp (DP-075, Morita, Tokyo, Japan) for 3 min while the another five samples without treatment were used as control.
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