Sentence examples for was analysed in triplicate from inspiring English sources
Each concentration was analysed in triplicate, and IC50 values were determined graphically from log concentration-inhibition curves (GraphPad Prism 4.03 software, GraphPad Software Inc .. Tacrine was used as a standard inhibitor.
DNA was analysed in triplicate using the Applied Biosystems 7500.
Two days later, the medium was refreshed and cells were incubated with the compounds to be tested dissolved in DMSO; each compound concentration was analysed in triplicate (final DMSO concentration is 0.2%).
The amount of immunoprecipitated DNA was analysed in triplicate using human ID-1 (sense: 5′-AGCTTGTGAGAGGCAGTGATTCT-′3; antisense: 5′-GGAACCCAGAGGTAGGCTGTT-′3) promoter primer sequences by real-time PCR with SYBR green PCR kit.
In order to measure the antioxidant activity of extracts, 10 μl of each sample at various concentrations (0.5, 2.5, 4.5, 7.5 and 9.5 mg/ml) was added to 990 μl of diluted ABTS+ and the absorbance was recorded every 1 min. We stop the kinetic reaction after 30 min. Each concentration was analysed in triplicate.
In order to measure the antioxidant activity of extracts, 10 μl of each sample at various concentrations (0.5, 2.5, 4.5, 7.5 and 9.5 mg/mL), were added to 990 μl of diluted ABTS solution and the absorbance was recorded every 1 min. We stopped the kinetic reaction after 30 min. Each concentration was analysed in triplicate.
Adjacent sets of unfixed rat brain sections were analysed in triplicate for quantitative autoradiography for TSPO with 125I]-CLINDE, OX-42 (microglial activation) immunohistochemistry and cresyl violet (cell loss).
The freeze-dried samples and reference materials were analysed in triplicate (3 mg) by heating at 50 °C for 45 min initially to remove residual moisture left in the sample and this was followed by heating to 105 °C in alumina crucibles at a heating rate of 20 °C min−1 in nitrogen (BOC, UK) at a flow rate of 50 mL min−1.
A total of 24 neonatal M. sexta larvae per plate per dose were analysed in triplicate, and five different doses were assayed.
Samples were analysed in triplicates.
Each cDNA pool was itself analysed in triplicate.
