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The complete cytb was amplified using Thermocycler GeneAmp 9700, USA.
The cassette from p426GPD-YAP1 wamplifiedied using primers GPD-YAP1-F and CYC1t-YAP1-R.
The target DNA was amplified using the species-specific primer sets (Table 1).
However, no DNA fragment was amplified using the specific primer of Pediocin PA-1 (Table 7).
The mtDNA D-loop region was amplified using the primer set designed by Meneses.
The 5′-flanking region of PhaPR1 was amplified using the GenomeWalker™ Universal kit (Clontech).
The coding region of the ClPDI cDNA was amplified using gene specific flanking primers.
The coding region of the TcAAD cDNA was amplified using gene specific flanking primers.
The coding region of the TcTrx cDNA was amplified using gene specific flanking primers.
The DNA was amplified using the primer pair, LCO1490 and HCO2198 (Folmer et al. 1994).
The 16S rRNA gene was amplified using GeneAmp 2700 PCR systems (BioRad, USA).
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