Transfection efficiency was always monitored by introducing pEGFP-C1 in parallel and examining the green fluorescence microscopically.
Rectal temperature was always monitored at the same time (10.00 A.M ., to avoid fluctuations due to defferent metabolic conditions.
The mRNA expression of the housekeeping gene hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) (NM_013556.2, 5'-GTC CTG TGG CCA TCT GCC Tand' and 5'-GGG ACG CAG CAA CTG ACA TT-3') was always monitored as internal control.
(Laurie Anderson, for example, had grown up only miles away!) I was always monitoring, with the most sensitive and well-calibrated apparatus, the degree of selloutitude exemplified by any given artist — musical, theatrical, whatever.
"We were always monitored, it's not that they needed to read the screenplay".
There are no snakes or dangerous animals in there because the perimeters are always monitored.' Nancy was in the show from the start and Vic was later introduced as a surprise guest.
"And if we are always monitored, always watched, always recorded, we're going to be much more reluctant to experiment with controversial, eccentric, weird, 'deviant' ideas — and most of the ideas that we care about deeply were once highly controversial".
But if our thoughts are always monitored and programmed by others, then how do we build our own judgment, our own sense of who we are and our personal integrity?" There is such a thing, Dr. Buchholz said, as too much stimulation.
We must teach our children everything under the sun starting from Day 1. "But if our thoughts are always monitored and programmed by others, then how do we build our own judgment, our own sense of who we are and our personal integrity?" But she pointed out that the overstructured life seems to be more of a problem for affluent children than for poor children.
The severity and frequency of symptoms and the global functioning and quality of life, as reported by caregivers, must be always monitored during treatment.
Children should also be always monitored for neurological and neuropsychological outcome to ensure that they get the necessary support for rehabilitation.
