There are also stringent accreditation and validation procedures in place for language schools in other countries.
Our target prediction criteria and methods were also stringent, but still allowed us to capture most miRNA targets that are conserved across several plant species, including Arabidopsis [ 48], poplar [ 17], rice [ 49], wheat [ 50], soybean [ 51], mustard [ 52] and grape [ 53].
It is possible that 33° was also too stringent to recover mutants in components of the Set1 protein methylation complex that methylates Dam1, which were previously shown to suppress ipl1 -2 (Zhang et al. 2005; Latham et al. 2011).
Enrichment analysis was also calculated for stringent sex-independent genes, which were selected to exclude genes showing weak sex-specificity at 8 wk (see Materials and Methods).
The nar operon, which encodes the respiratory nitrate reductase in Brucella, was also induced under stringent conditions, as evidenced by the microarray analysis and by dosage of the nitrites produced by the nitrate reductase.
Protection was also defined by very stringent criteria: complete lack of evidence of infection, by any method of detection at any of the time points analyzed, including a systemic post-mortem analysis.
Subsequently, the taxonomic classification of reads was also made with less stringent setting (Min Support: 1, Min Score: 10, Min Score/Length: 0.00, Top Percent 100).
Also, this study was also limited by its stringent inclusion and exclusion criteria, which were necessary in ensuring safe administration of nortriptyline, but which excluded a high proportion of men interested in quitting smoking.
