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During pretreatment, a significant amount of lignin was also solubilized into the liquid stream, causing the lignin reduction in the pretreated solids.
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Other non-toxic metals such as Ca and Mg, that are present in important concentrations in this soil, are also solubilized, but important differences in the behavior of each metal are observed.
Other elements present originally in the concentrate such as Zn, Sb, and Cu were also solubilized.
However, other sludge components such as NOM are also solubilized in acid, both increasing the acid requirement and contaminating the recovered coagulant.
DTT, a strong reducing agent, was used to partially disintegrate the nematocyst capsule and cause venom release, so it is likely that a proportion of capsular components and other structural proteins were also solubilized during this process.
Direct growth of polymer brushes from exfoliated graphene surface was also used to solubilize graphene in desired solvents.
The Zn2+ effect was also demonstrated for CusA solubilized in C12E8 and C12DAO (not shown).
Another zwitter-ionic detergents n-Dodecyl-β-iminodipropionic acid, monosodium salt (#79) was also reasonably effective to solubilize Trx-hCRs.
According to the Klason lignin determination (Table 2), 4% of the acid insoluble lignin was solubilized, which was also visible in the UV spectra of the liquid fractions as an increase of the absorbance at 245 to 295 nm compared with the control.
Additionally, a mixture of 0.1% DDM and 1% polyoxyethylene-8-dodecylether (C12E8) that was successfully used to solubilize Sav1866 [17], was also tried but did not give any improvement as compared to DDM alone (not shown).
The vehicle that is saline used for solubilizing the NHAG was also tested, and we did not observe any effect of saline on the concentration of the measured cytokines.
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