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The material decomposition was allowed to differentiate between liver enhancement of CA1 and CA2, as shown in Figs. 4 and 5, where we show the distribution of both CAs within the liver parenchyma, in addition to a conventional CT image, for comparison.
To explore early granuloma formation in the lung tissue model, Mtb-infected macrophages and uninfected PKH26-labeled monocytes were introduced simultaneously at a ratio of 1 5 and the tissue was allowed to differentiate during air exposure, resulting in stratification of the epithelium (Nguyen Hoang et al., 2012).
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For differentiation analysis, PNS and SNS were allowed to differentiate on poly-L-ornithine/fibronectin-coated coverslips for 5 days, followed by immunocytochemistry.
Cells were allowed to differentiate into myotubes by replacing the growth medium with differentiation medium (DMEM with 1% heat-inactivated horse serum, 100 µg/ml streptomycin sulphate, 100 units/ml penicillin-G) in 18% CO2 for 5 days.
To obtain differentiated hypothalamic neurons culture, the plated neurospheres were allowed to differentiate for 18 days in Neurobasal medium with 500 µM L-Glutamine, 2% B27 supplement, 100 U/ml penicillin and 100 µg/ml streptomycin (all from Gibco), with no growth factors.
Growth media (GM) was replaced with differentiation media (DM) 19 h after transfection and cells were allowed to differentiate for 96 h.
After further two days, PM was replaced by differentiation medium (DM ; 2% FCS in DMEM) and cells were allowed to differentiate for 2 or 5 days.
Myoblasts of the murine cell line C2C12 were allowed to differentiate in a low serum containing media and myogenesis assessed by muscle specific protein expression.
After H9 cells were allowed to differentiate for 2 weeks, both α-fetoprotein (350.9 ± 14.2 IU/ml) and human chorionic gonadotropin (hCG, 46.7 ± 5.6 mIU/ml) were detected in conditioned culture medium, indicating endoderm and trophoblast differentiation (20).
Following sorting, cells were allowed to differentiate for 2 3 weeks prior to immunostaining and electrophysiological recording.
Cells were allowed to differentiate to the chemotaxis competent stage as described previously [32], [33].
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