Sentence examples for was allowed to bind from inspiring English sources

32P-labeled (1 pmol, 20,000 cpms) IDX-rasISS1 RNA was allowed to bind to 640 ng recombinant hnRNP H in binding buffer (1 mM ATP, 1.7 mM MgCl2 and 0.1 µM heparin in 1×Roeder D buffer).

5 ug of nuclear protein was allowed to bind to a 32-P labeled, double stranded, oligonucleotide encompassing the NF-κB binding site of the HIV-1 LTR (5'-ACAAGGGACTTTCCGCTGGGGACTTTCCAGGG-3') at room temperature in the presence or absence of antibodies specific for the NF-κB transcription factors p50 or p65 (Rel A) (Santa Cruz).

The supernatant was added to a Ni2+-NTA resin column (1 ml bed volume) equilibrated with lysis buffer and was allowed to bind slowly.

Horseradish peroxidase conjugated streptavidin (0.1 µg/ml) was allowed to bind to the Biotin-PNA conjugates.

Then the virus (m.o.i. of 5) was allowed to bind to the cells for 30 min at 4°C.

The cell lysate was allowed to bind to 30 µl of MagneHIS (Promega #V8565) with shaking at 900 rpm for 20 min (10 min clockwise, 10 min counterclockwise).

The ligand binding assay was a variant of the ELISA in which dilutions of ligand (rhFGF-2) were allowed to bind to a ligand binding protein such as rhPln.D1 that had been coated onto the wells in PBS-N3.

The biotinylated peptides (20 pmol in 50 µl ddH2O) were allowed to bind the streptavidin-coated high-capacity binding plates (Pierce #15,503, Rockford, IL) overnight at 4°C or 2 hr at room temperature.

After addition of a binding buffer and the magnetic particles (Siemens Healthcare Diagnostics, Cologne, Germany) nucleic acids were allowed to bind to the particles for 15 min at room temperature.

Then the mixtures were allowed to bind to glutathione-Sepharose.

Antiserum samples after different consecutive immunizations were allowed to bind to the immobilized antigen (BSA and ROS-BSA).