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The bioreactor was agitated at 350 rpm using a Rushton turbine agitator.
Then, 5 μL of IFN-γ catch reagent and 2.5 mL of warm (37°C) RPMI 1640 containing 10% human serum were added to the blood samples; the mixture was agitated at 90 rpm for 2 hours at 37°C (agitation is crucial to avoid IFN-γ capture by non-secreting cells).
In order to investigate if particle size reduction was caused by the physical effects of agitation rather than enzymatic action, spruce material at 13% WIS was agitated at 600 rpm without enzyme addition.
The experimental setup was agitated at 180 rpm at 35 °C.
Every flask was agitated at different time intervals (30 960 min).
The mixture was agitated at 120 rpm at 30 °C until equilibrium was reached.
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The sample was then heated at 80°C for 10 min while being agitated at 500 rpm in an agitator of autosampler of GCxGC TOF-MS.
…The subject of the division of the state has been agitated at one time and another ever since I can remember".
Cell suspensions were agitated at 120 rpm without aeration.
The mixtures were agitated at approximately 200 rpm with a magnetic stirrer.
The solutions were agitated at 150 rpm for a predetermined period at 30 °C in a shaking incubator (JEIO TECH SI-900R).
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