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When Christopher died childless in 1448, the Scandinavian union was again dissolved.
The Boeotian League was again dissolved, and after an abortive revolt (335) against Alexander the Great of Macedon, Thebes and the rest of Boeotia fell permanently under external domination.
This was again dissolved, and the polonium electroplated on platinum foils.
Coke was notably absent from any preparations for defence, feeling that the risks of invasion were exaggerated, but was eventually persuaded by public opinion to reform the Yeoman Cavalry in 1803; it was again dissolved in 1805.
The pellet was again dissolved in 1.5 mL PBS.
The precipitate was again dissolved in water and added to ethanol.
Similar(54)
The precipitates were again dissolved to acetone.
Pure plasmid DNA was again ethanol precipitated, and the pellets dissolved in 10 mM Tris pH 8.0.
The following day 10 µL of the dissolved plasmid (1µg) was again lyophilized to remove the solvent, and then resuspended in 4 µL TE buffer for plasmid digestion with either EcoRI (2 µL) or PstI (2 µL) for 4 h at 37°C followed by gel electrophoresis.
The league later opposed Sparta in the Corinthian War (395 387) and was defeated and again dissolved, Sparta having had Persian help.
The mixture was again evaporated to dryness and the residue dissolved with 0.5 mol L-1 HNO3.
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