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It had been developed for an appearance on Heston's Feasts, and was afterwards added to the menu at the restaurant.
This mixture was afterwards added to 50% confluent PK-15 cells in a 96-well plate.
The precipitate was afterwards added 3 μL alcalase (Novozymes A/S) per mL of 100 mM sodium-citrate buffer, pH 7 at 5% DM, stirred in an incubator at 50°C for 20 h and cleansed by three repetitions of centrifugation and washing in MQ water.
Similar(57)
What doesn't kill us makes us stronger," Ancelotti smiled afterwards, adding: "I'm joking: I'm not a philosopher.
"He's very good with the ball at his feet," said Villas-Boas's assistant, Steffen Freund, afterwards, adding: "It's nice to see the young players doing so well".
But K3's "Rules Manager" offers a GUI interface that lets end-users customize their own "if/then" statements for how the data needs to be enriched afterwards (add this reference, set this field, e.g).
– is obscured by branches (was that really the sound of the caiman's skull crunching or was it added afterwards, by the way?).
Propidium iodide (PI; 1 μg/ml) (Sigma, UK) was added afterwards, and all samples were analysed within 1 hr of PI staining using FACSCalibur (BD, Oxford, UK).
Complete medium was added afterwards and the cells were incubated at 37°C, 7.5% CO2 for 30 minutes.
Afterwards, alcohol was added to the solution and the precipitate was separated by filtration and dried at 50°C in a vacuum oven.
It might even be slightly out of tune with the rest of the song, since it was added afterwards.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com