After the potential was adjusted to zero, the bath solution was replaced with Ringer's solution.
Mature AGS cultivated in the SBAR was kept at a 4°C refrigerator, and pH was adjusted to 7.0 7.2; especially, the feed liquid was replaced every two weeks.
The pH of the nutrient solution was adjusted to 5.5-6 using 50% phosphoric acid, and the solution was replaced every two days.
The solution was replaced once a week, and the pH was adjusted to 5.2 every day.
Nutrient solutions were replaced every three days and initial pH value was adjusted to 6.3 with 1.0 mM NaOH.
In solutions designed to produce metabolic acidification, 20 m m NaCl was replaced by 20 m m sodium l-lactate, and pH was adjusted to 6.7 with NaOH.
The colloidal solution solvent (benzyl alcohol) was replaced with isopropanol using a rotary evaporator and the concentration of ZnO was adjusted to 0.2 wt% [25 28].
The colloidal solution was replaced by isopropanol from water using a rotary evaporator and the concentration of ZnO was adjusted to 0.2 wt% [25 28].
The numbers in Table 7 can easily be adjusted to other conditions or replaced if better estimates or assumptions are available.
Experimental diets in this category were adjusted to control diets by partly replacing the carbohydrates and/or soy oil by protein and leucine (151 g casein/kg and 16 g leucine/kg feed; TB+hpr+leu), high protein and fish oil (151 g casein/kg and 22 g fish oil/kg feed; TB+fo+hrp), or high protein and leucine and fish oil (151 g casein/kg, 16 g leucine/kg and 22 g fish oil/kg food; TB+fo+hpr+leu).
For each call, pitch floor and ceiling variables were adjusted to surround the observed fundamental frequency, replacing standard settings.
