The intrinsic region of type 3 samples is similar to that of type 2, but every 10 layers of Ge quantum dots are additionally separated by 100 nm of Si.
Only for sample containing 30 layers of 6 monolayer Ge quantum dots separated by 5 nm silicon layers in which every 10 layers of Ge quantum dots were additionally separated by 100 nm of Si (type 3) was observed two peaks on the temperature dependences of conductance.
Patients in the INCO group were additionally separated in patients who had received any botulinum toxin treatment before study start ('BoNT/A-pretreated') and those who were BoNT/A naïve, since pretreatment may have an effect on the efficacy of the study treatment.
Substances were isolated by reversed phase chromatography; in some cases isomers were additionally separated by chiral chromatography on Chirobiotic T. When tested on human recombinant melanocortin receptors all the tertiary amides showed some binding affinities; for the highest affinity compounds the Kis reached 400 nM on MC1, 2 μM on MC3 and 1 μM on MC4 and MC5 receptors.
When pDC were excluded and DC were additionally separated into conventional subsets, however, weak, but reproducible responses were seen for Langerhans and dermal DC (Figure 3C,D).
Both current components were additionally separated pharmacologically by application of 10 mM 4-AP to proliferating hNPCs, with IK being identified as the 4-AP-insensitive component measured in activation protocols and IA was isolated by subtracting the 4-AP-insensitive component of steady-state inactivation currents from control currents (Fig. 1).
When xylose and galactose were present in the same sample xylose, galactose, glucose and xylitol were additionally separated and quantified by one or two in series connected HPX-87P (Bio-Rad, Hercules, CA, USA) (Waters, Milford, MA, USA) ion exchange column(s) at 85°C with water as the mobile phase at a flow rate of 0.5 ml min-1.
The trochlea of the femur, the central segment of the femoral condyle, and the posterior segment of the femoral condyle were additionally separated into one internal and one external segment by using 50%% of the maximum medial to lateral extent of the segmented subchondral bone area as criterion (Fig. 2a- c).
This was additionally supported by separate secondary structure prediction for individual sub-groups with potentially homologous regions such as the ComEA N-terminal regions and the polysaccharide export proteins.
The layers were separated and the aqueous phase was additionally extracted with DCM.
