Supplemental water was added, when permissible, if rain levels fell below one inch every seven days.
Soon after, another restriction was added when a group that met for discussions about Islam was told to stop.
It was added when, in March, New Line, the production company, shot extra footage to juice up the thrills.
Only the shepherd's pie is on the regular menu and that was added when regular customers requested it.
Sigma Antifoam 289 was added when required.
If the induction was needed, the inducer was added when OD600 was at 0.3.
Sodium phosphate dodecahydrate (Acros Organics, 8 mM) was added when noted.
Diluted antifoaming agent was added when foam appeared in the fermentor during the fermentation process.
Ampicillin was added when required at a final concentration of 80 µg/mL.
Organic solvent was added when the biomass reached 0.5 OD, in order not to affect cell growth.
Substrate 2-cyclohexenone or xanthine (5 mg, 0.05 mmol, final concentration 50 mM) was added (when xanthine was used, DMSO was used to improve the solubility).
