Once the sample was dry and cooled down, 100 μl of perchloric acid and 15 mL of double-distilled water were added, transferring the final volume into a voltamperometric cell.
"Advisers complement and supplement the work that has to be done, but they also ensure that there's added transfer of skills and capacity building, things which sometimes get missed," says O'Keeffe.
Following shaking, incubation and centrifugation, the upper aqueous phase was transferred and 900 μL ethanol (Acros Chemical) was added and transferred to the RNeasy MinElute column.
After vortex mixing and centrifugation, the lower phase of the vial, to which H2O was added, was transferred to a new Wheaton vial, and the lower phase from the CHCl3 re-extraction was transferred to the vial containing the residual H2O phase.
Immediately after the pulse, 1 ml of ice -cold 1M sorbitol was added and transferred into a tube containing 4 ml of YC-trp media for incubation at 30°C.
After 10 min incubation, the Grace's Insect Cell Culture Medium supplemented with 10% FCS was added and transferred onto the cells.
Approximately 500 μl of buffer RBC was added and transferred to a DNA column, centrifuged, and the flow-through was collected.
Stirring continued for an additional 2 min after the last water droplet was added, before transferring the high internal phase emulsion (HIPE) to a 50 mL centrifuge tube.
3 μL of a 0.1% fibronectin solution (Sigma-Aldrich, Oakville, ON) was added before transferring the complexes to a 384-well plate.
Next PBS (1 ml) was added and cells transferred to centrifuge tube (15 ml).
