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BIO was added to developing amphioxus embryos at blastula stage and embryos were allowed to develop until mid-neurula stage at which point mRNA was isolated and gene expression interrogated by real-time quantitative RT-PCR.
Torin1, a direct inhibitor of mTOR kinase activity (Thoreen et al., 2009), was added to developing zebrafish embryos at 24 hours post fertilization (hpf) and survival was assessed daily.
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Hydrazine was added to develop nanoparticle formation in the core of the micelles by reducing the metal oxides.
To 1 ml of supernatant, 5 ml of 20 µg/ml of 2,6-dichlorophenol-indophenol (DCPIP) dye was added to develop color.
was added to develop the reaction.
NTB/BCIP (Sigma) was added to develop the color reaction.
Finally, fresh DAB and H2O2 (Thermo Scientific (Erembodegem, Belgium) solution was added to develop the colored spots.
A systematic search on Medline was added to develop best estimates on incidence and prevalence [ 21- 29].
The substrate diaminobenzidine (DAB; Peroxidase Substrate Kit, Vector Laboratories Inc). was added to develop the slides.
After washing 4 times, 100 ul TMB substrate was added to develop color.
Briefly, 2 ml of chloroform was mixed with 5 ml (1 mg/ml) of each sample in a test tube then 3 ml of concentrated H2SO4 was added to develop the color.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com