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Finally, in order to ensure that the statistician was also blinded to treatment arms when analysing study data, an additional level of blinding was added subsequent to trial completion.
Moreover, if NIH-12848 was added subsequent to dome formation, the domes disappeared with a detectable effect within 3 h that was maximal by 24 h, indicating a dynamic relationship between PI5P4Kγ and the development and maintenance of this morphology.
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Interestingly, several elements contained in the left bar and in the back of the chair, including cadmium, cobalt, chromium and calcium, were not detected from the right chair stile, suspected of being added subsequent to the date of the painting execution.
The ATP synthase inhibitor oligomycin (oligo), the uncoupler FCCP, and the electron transport chain inhibitor antimycin A (AA) were added subsequent to RK-33.
At 4th day adipocyte differentiation media was added by subsequent changes in every 2 days.
In both these studies, dactinomycin was added to subsequent infusions in patients with resistant disease.
After incubating for 30 minutes, HRP substrate was added and subsequent steps were as for the NN ELISA.
At 24 h post-transduction, the virus-containing medium was removed and fresh medium was added every subsequent day.
After 1 min of mitochondria preincubation, 0.7 μM BTM-P1 was added and subsequent oxidation of endogenous NAD(P H was observed.
Using size-fractionation, small RNA (<300 nt) enrichment was performed, following extension with a poly(A) tail, to which the an oligonucleotide tag was added for subsequent labelling.
Then, a cytokine mixture (rat or human IFN-γ 1,000 U/ml + IL-1β 100 U/ml + TNF-α 1,000 U/ml) was added for subsequent 48-h incubation.
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