0.1×MMR was added and properly dividing embryos were selected and screened for GFP fluorescence as described above for F0 tadpoles.
Then, the alkaline solution of required quantity was added and mixed properly by hand covered with gloves to eliminate clustering.
Commercially available lyophilized Altepase (Streptokinase) vial (Beacon pharmaceutical Ltd., Bangladesh) of 15,00,000 I.U. was collected and 5 ml sterile distilled water was added and mixed properly.
To the commercially available lyophilized Streptokinase (SK) vial (Durakinase, Dongkook Phama. Co. Ltd, South Korea) of 15 00000 I.U., 5 ml sterile distilled water was added and mixed properly.
To the commercially available lyophilized SK vial (Polamin Werk GmbH, Herdecke, Germany) of 15, 00,000 I.U., 5 ml sterile distilled water was added and mixed properly.
Commercially available lyophilized Streptokinase vial (Polamin Werk GmbH, Herdecke, Germany) of 15,00,000 I.U., 5 ml sterile distilled water was added and mixed properly.
After that,.5 mL of 80% KOH was added for saponification and properly vortexed before putting it back to 85 °C water bath for 10 min.
After five minutes, 10 ml of 7%Na2CO33 solution was added to the tube and mixed properly.
The medium was then discarded and 200 μL of DMSO (dimethyl sulphoxide) was added to the wells and mixed properly to dissolve the formazan crystals.
Sterile PBS (2 ml) was added to the 2 ml of blood and properly mixed.
