Sentence examples for was added placed from inspiring English sources

The resin was washed with 200 ml of acidified water (5% acetic acid) and 1 mL of the blue corn or tortilla crude extract was added placed and washed with 100 mL of acidified water; the polymer mixture was eluted with 200 mL of acidified ethanol (5% acetic acid).

50 seeds were placed evenly on filter paper within 9cm Petri dishes to which 4.5 ml 5-AzaC solution (or water, for controls) had been added, placed in a containment tray and incubated in the dark at 20°C for three days.

Supernatants were transferred to a new tube and ice-cold acetone was added, and placed at −20 °C for 1 h.

Then 0.5 ml of 0.75% TBA was added and placed in a water bath for 45 min at 80 C.

The membrane was washed a third time with TBST then ECL liquid was added and placed in a darkroom.

4 mL 99.5% formamide per gram of tissue was added and placed on a shaker for 48 hours.

A second cell lysate served as an undigested control; 120 U of SUPERase-In was added and placed on a nutator as above.

To this 1 μl of Oligo (dT 18 primer was added and placed at 65°C for 10 minutes and then at room temperature for 2 minutes.

sulphuric acid was added and placed in water bath for 10 min at 100°C to convert the PHB into crotonic acid.

To the fermented medium, 20 mL of double-distilled water was added and placed in an orbital shaker at 150 rpm for 30 min.

The Superscript mixture (GIBCO BRL, Paisley Scotland, UK) was added before being placed at 37°C for 1 h.