Exact(2)
250 μL of this extract was added into one ml 0.25%% (w/v) periodic acid/0.25 % (w/v) meta-periodate and incubated at 37 °C for 15 minutes.
The cells were split into two tubes and 2 mM (final concentration) IPTG was added into one of the tubes.
Similar(58)
Then 15, 25, 50, 100 μg/ml SWCNTs solution were added into one well, and a control well without SWCNTs was set up.
Three leaf discs (6 mm in diameter) from the third fully expanded leaf were added into one tube containing 100 μL of distilled water.
A total of 400 larvae of both molecular forms (200 M: 200 S) were placed into each cage and predators found in situ, were added into one cage whereas the other remained predator free.
For this reason, regulation on menstrual cycle is needed to treat PMS for TCM doctors and that is why many CHM other than JWXYS are added into one prescription for PMS.
10 µg/ml bovine insulin (Sigma) was added into the medium of one of these groups.
One gram of faeces was added into a rest tube containing one mL of 24% (v/v) metaphosphoric acid which was diluted in sulfuric acid 3 N.
Glutamine supplementation of 20 g was added into the all-in-one bag containing 2,000 kcal used for parenteral nutrition.
Then the purified TsCl (1.05 equiv, 2.0 g; 10.05 mmol) was dissolved in 20 mL methylene chloride, and the solution was added into the above PEG solution in one portion under rapid stir.
Then 20 μL of Cell Titer 96 Aqueous One Solution Reagent was added into each well, and MTS reduction was measured 2 h later spectrophotometrically at 492 and 690 nm as background using μQuant equipment (Bio-Tek Instruments, Winooski, VT, USA).
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