Exact(1)
A total of 3.7 g of sodium bicarbonate was added, dissolved with 1 L of ultrapure water and the pH of the medium was adjusted to 7.0.
Similar(59)
Finally, the solution from each well was aspirated, and 150 μl of dissolving agent was added to dissolve the formazan precipitate.
Thereafter 0.267 g of sodium deoxycholate was added and dissolved in the solution.
After 4 h of reperfusion, 100 μL of DMSO was added to dissolve the formazan crystals.
Then the medium was removed carefully, and 100 μl DMSO was added to dissolve formazan crystals.
After cooling, 4 ml of 1.5% hydrochloric acid was added to dissolve the residue.
Then the supernatant was removed and an equal volume of DMSO was added to dissolve the formazan crystals.
The medium was then aspirated, and 100 μL of DMSO was added to dissolve the formed formazan crystals.
After incubation for 4 h, 150 μl of DMSO was added to dissolve the non-soluble crystals.
In the case of KO2, an excess amount of 18-crown-6 was added to dissolve the KO2 and thus enhance its reactivity.
MTT was aspirated off, and DMSO was added to dissolve the formazan crystals.
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