Sentence examples for was added developed from inspiring English sources

Substrate [p-nitro phenyl phosphate, PNPP, (Bio-Rad, 170 1063)] was added, developed for 30 min and optical densities determined at a 405 nm wavelength using a microplate reader and SoftmaxPro software.

Then anti-Dig-ALP antibody was added and developed using PNPP solution as mentioned above.

Then, 80 μl of five times diluted Malachite green reagent was added and developed for 60 minutes at room temperature.

The appropriate biotinylated antibody for each cytokine was used and streptavidin-HRP was added and developed using 3,3′, 5,5′-tetramethylbenzidine (TMB) substrate solution.

Then 1 5000-10,000 diluted peroxidase-conjugated sheep anti-rabbit or mouse IgG antibody (Amersham, United Kingdom) was added and developed using the SuperSignal West Dura Substrate (Thermo Scientific, Waltham, MA).

Hydrazine was added to develop nanoparticle formation in the core of the micelles by reducing the metal oxides.

To 1 ml of supernatant, 5 ml of 20 µg/ml of 2,6-dichlorophenol-indophenol (DCPIP) dye was added to develop color.

was added to develop the reaction.

NTB/BCIP (Sigma) was added to develop the color reaction.

In addition, a new section was added called 'Developing a drug use problem'.

Finally, fresh DAB and H2O2 (Thermo Scientific (Erembodegem, Belgium) solution was added to develop the colored spots.