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For the minus-RT control, water was added at this time point.
Other than a participant identification number, no other participant information was added at this time.
In some experiments, blocking anti-RANKL antibody (5 μg/ml; PeproTech EC) was added at this time.
We therefore determined using immunoblot analysis that gctBid is present after an extended time (17 18 h) and confirmed that cyt c was released once ABT-737 was added at this time, even if the GraB is no longer active.
For crosslinking for immunopurification (IP) experiments, 750,000 T84 cells were seeded into 6-cm tissue culture plates in 6 mL of media containing 100 μM Ac4ManNDAz; if required, a glycosylation inhibitor was added at this time.
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Alloys, which oxidize easily, are added at this time to minimize losses and to improve metallurgical control.
Additional Bios are being added at this time.
The north and south transepts were added at this time as well as the whole south aisle of the nave, the chancel, a clerestory and a new porch.
In addition, 8 triple and 26 single 25mm AA mounts were added at this time, increasing the total number of 127 mm and 25 mm anti-aircraft guns to 24 and 162, respectively.
Inhibitors may also be added at this time.
The culture medium was changed on days 4, 8 and 12, and fresh drugs were added at this time.
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