Exact(2)
At 2 hours (additionally, at 0 hours for primary human neutrophils), protease inhibitor cocktail (Sigma, #P1860) was add at 1 200 dilution.
Briefly, the adherent cells in 6-well plate were collected by 2 mM EDTA in PBS on ice, TCA was add at final concentration of 1% and vortex vigorously for 10 sec.
Similar(58)
Salt was added at 1%.
Chloramphenicol was added at indicated concentrations.
Pure resin was added at room temperature.
CsA was added at different times during this process.
The AOX was added at 0.025% in the ration.
Finally, butter was added: "At that point I got sick".
Another concert was added at the Zenith (capacity 6,500).
Subsequently, ASA was added at various concentrations.
Water was added at 50mL/1000 g feed.
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