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The recombinant CgChoA was active between pH 4 8 with optimal activity in the neutral range similarly to other cholesterol oxidases (Table 1), e.g. at pH 6.75 using 0.011 M MOPS buffer for the coupled HRP assay.
The purified enzyme was active between pH 7.0 and 9.8, and maximal activity of the enzyme was observed at pH 7.0.
The enzyme was active between pH 6.0 and pH 9.0 with maximum activity at pH 8.0 when incubated for 10 min at 60°C).
The enzyme was active between temperatures of 50 to 75°C, with more than 50% relative activity.
The pH activity profile reported in Figure 5(a) showed that the crude enzyme was active between pH 5.0 and pH 11.0 with an optimum at around pH 8.0.
INSIDE: The firehouse was active between 1896 and 1974, and sold at city auction in 1977 to its current owners.
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