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For region of interest identification, a T1-weighted MR image was acquired separately with a Siemens 3 T Trio scanner using an extremity coil and the following spin echo sequence: [echo time (TE) = 3.34 ms, repetition time (TR) = 2530 ms, flip angle = 7°, thickness = 0.50 mm, field of view = 140 mm].
For multicolor fluorescence images, each channel was acquired separately.
The A. lituratus data was acquired separately (McCulloch and Stevens 2011); phenol chloroform DNA extraction was used, and 454 Titanium sequencing was performed at Duke University Genome Sequencing and Analysis Core Facility using standard protocols.
Each wavelength was acquired separately by invoking frame lambda, and images were processed with Elements computer software (Nikon Inc .. Pathology and morphological changes, as observed in histochemically-stained sections, were qualitatively graded using a scale ranging from no detectable changes, to mild, moderate, or severe changes.
For property to be considered exempt from this rule, the deed must state the property was acquired separately.
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Positive and negative mode data were acquired separately.
MRI and 18F-choline PET/CT were acquired separately on average 21 days apart (range 0 47 days).
The performances of the fabricated EDFRR were measured and the experimental Q-factor of 2.47×108 and resonant depth of 109% were acquired separately.
Absolute thresholds were acquired separately for 1 kHz and 0.5 kHz pure tones and broadband noise using a 1-up 1-down adaptive staircase before any of the noise conditions were run.
To study mutual orientation of the GA and the initial contact expansion, cells were pre-incubated with the GA fluorescent probe brefeldin-BODIPY558 (Molecular Probes) at 0.1 µM for 20 min. Three-dimensional images were acquired separately on the wavelength corresponding to the fluorescence of the GA label, and in transmitted light showing the area of cell contact with the substrate.
In contrast, several hydrolases appear to have been acquired separately by one or two Frankia strains.
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