Exact(2)
One hundred microliters of warm fixation solution containing 8%paraformaldehyde/8%% sucrose in PBS++ were added to each well of a 96-well plate.
The plates were incubated at 37°C for 30 minutes with agitations (warm fixation) and results were read after the plates have been centrifuged at 2500 rpm for 5 minutes at 4°C.
Similar(57)
These both methodologies can be influenced by pre-analytical factors including warm ischemia, fixation time and tissue processing conditions.
Adhesion was blocked by washing cells twice with warm medium before fixation with 4% (w/v) paraformaldehyde, staining with Crystal Violet [0.5% in 20% (v/v) methanol] and solubilization with 1% SDS.
An aliquot of the infected cell suspension was carefully spotted onto each well of Teflon coated slides, followed by air-drying over a warm plate and subsequent fixation in cold acetone for 10 min. Serial 2-fold dilutions of serum samples were then added to detect specific reactivity.
In terms of eco-efficiency, the LCF biorefinery shows better environmental performances mainly in global warming potential due to CO2 fixation during acid fermentation.
Internbational fixation on global warming is also motivating much of Israel's new international technological development.
Subsequent geographic isolation of populations during Holocene warming probably led to the fixation of local sequence types.
Previous work that had been carried out to ensure methodological precision [ 2, 3] regarding palpation procedures, skin fixation pad placement, warm-up/familiarization movements and a standardized neutral starting position, were all part of the methodology of this multi-rater study, and may have contributed to the levels of agreement achieved by the operators in this particular investigation.
Monolayers were wounded with an injection needle, rinsed with warm DMEM, and incubated in DMEM before fixation with 3.7% paraformaldehyde (PFA).
How else to explain this silly fixation on 1998 being the warmest year?
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