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The biggest disappointment was that when the huge glass wall was fixed, the original five-meter-wide sheets were replaced with smaller sheets joined with clear silicone.
In addition, the bottom wall was fixed, while the top wall had a velocity to apply a displacement load.
The aneurysmal wall was fixed in paraformaldehyde (3.7%) for immunohistochemical analysis or incubated for 16 hours in RPMI-1640 at 37°C (Invitrogen, Cergy-Pontoise, France) (6 mL/g of wet tissue), in order to obtain conditioned medium.
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The vapor split at the bottom of the wall is fixed at design by the location of the wall.
It is shown that the internal structure of the wall (the number of air caverns) can be optimized so that the overall thermal resistance of the wall is maximal, while the mechanical stiffness of the wall is fixed.
On the front wall is fixed a wooden board, the upper edge of which, 0.68 m from the floor, constitutes the play line; 2.93 m from the floor a second line called the cut line or service line is marked.
Biofilms formed by adherent 'sessile' organisms in plate wall were fixed with sodium acetate (2 %, w/v) and stained with crystal violet dye (0.1 %, w/v).
Tissue specimens from the antral wall were fixed in Histofix (Histolab Products AB) and embedded in paraffin.
Fragments of the LV wall were fixed in formaldehyde solution 10%, buffered (pH 7.2) for 48 h, embedded in paraffin, and used for the histological slices, 6 μm thick, which were analyzed through polarized light microscopy with the use of Picrosirius staining in order to study the interstitial myocardial collagen fibers.
To study the differences in wall topography before and after the percolation test, the walls were fixed in a support enabling their identical repositioning before and after the percolation test so as to minimize the map rotations and translations necessary for a good superposition.
The wall thickness was fixed to its known value (1.2 mm).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com