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Likewise, plant cell walls are a primary source of nutritional energy for ruminants, but with many forages less than 50% of the cell wall fraction is digested [ 7].
10 kDa heat shock protein found in cell wall fraction is an important antigen recognized by T-cells, also known as chaperonin-10 (cpn-10).
However for many types of forage, less than 50% of the cell wall fraction is digested and utilized by the ruminant host [ 1].
Plant cell walls represent a major source of nutritional energy for ruminants, but unfortunately with many types of forage, less than 50% of the cell wall fraction is readily digested and utilized by the ruminant host.
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The major cell wall fraction was the AS.
As shown in Figure 2A, the LevQ signal was only found in the cell membrane fraction and the cross-reactive protein in the cell wall fraction was not derived from LevQ (Figure 2A) or from the lysozyme/mutanolysin cocktail used to digest the cell walls (Figure S5).
First, the cell wall fraction was prepared to minimize intracellular contamination and loss of proteins [ 14].
Moreover, the soluble cell wall fraction was significantly lower in the TFM7-OE fruits than in WT or TFM7-CS.
The cell wall fraction was then washed three times in sterile water, re-suspended in DMSO and stored at −80°C.
The supernatant (cell wall fraction) was directly mixed with 50 µL of loading buffer for PAGE analysis.
Fibre wall fraction was obtained by multiplying mean fibre wall proportion in a fibre by the fibre fraction per cross-section.
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