Sentence examples for walking method from inspiring English sources

The two-step gene walking method consisted of a walking-PCR (step 1) followed by direct sequencing of the PCR product (step 2) [ 17].

Fig. 1 The genome walking method scheme.

To amplify the flanking sequences that are adjacent to Tn5 transposon insertion sites in Serratia marcescens FZSF02, we developed a genome walking method based on TAIL-PCR.

In the present work, a PCR based genome walking method was adopted to isolate and clone the promoter for the NMT gene.

Near full-length genome of the construct was sequenced using primer walking method described previously [6].

The primer walking method was used to finish insert sequencing, with primers automatically designed by PRIDE [23].

These newly developed DNA walking methods are completing the previously implemented DNA walking method targeting the t35S pCAMBIA element.

Previously, we proposed a walking method named the "leg-grope walk" method, and discussed the validity of this strategy based on our experiments on a fragile horizontal plane [21].

using the Primer-Walking method.

The genome-walking method has attracted some attention.

Then, a contiguous sequence extended to 3,200-bp 3,200-bps obtained by gluome-wasking methobtained