Sentence examples for walker kit from inspiring English sources

The Clontech Universal Genome Walker kit was used to obtain the 5′ and 3′ ends of the ORF, following the manufacturer's protocol.

Introns and flanking genomic regions were isolated using PCR with specific primers on a genomic library created with the Genome Walker kit (Clontech).

The genome walking was performed as a nested hot-start long-range PCR (conditions available upon request) with the five human templates from the Genome Walker kit #K1803-1 (Clontech, Palo Alto, CA, USA).

A 3,561 bp fragment from the 5′-end (promoter) region of PvTIFY10C (Phvul.002G002000.1) was cloned using the Universal Genome Walker kit (BD Biosciences, San Jose, CA, USA), following the manufacturer's protocol.

Arbitrary primers (ARB6 and ARB2) and a specific KS10 primer, in addition to the APA gene Gold genome walker kit (Bio S&T, Montreal), were used in PCR experiments to determine the precise insertion site of the integrated prophage.

For the isolation of the 5' sequence upstream of the BjMTP1 translational start site we used a Universal Genome Walker kit (Clontech, Mountain View, CA) following the manufacturer's protocol.

The resulting fragments were blunt-end ligated to the Genome-Walker Adaptor provided by the Genome-Walker kit (Clontech) to generate the corresponding libraries.

Laura Freeman, in the Daily Mail, advised readers that "Weatherland should be as essential a part of any walker's kit as a balaclava, sun hat and pac-a-mac".

The Universal Genome Walker™ Kit (Clontech Laboratories, Inc., Palo Alto, CA, USA) was used to isolate the promoters regions of Ppbec1, Ppxero2, Pptha1 and Pplox1.

"Decent footwear is the most important piece of kit for any walker," says Dominic Bates, editor of Walk, the magazine of the Ramblers.

There is little doubt that Medicare has been paying far too much for equipment — including wheelchairs, hospital beds, oxygen concentrators, diabetic test kits, and walkers — under fee schedules based on historical charges.