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Cell debris was excluded on the basis of forward vs side scatter.
Forward vs side scatter gating was determined using human blood platelets as controls.
Debris was eliminated by gating on forward vs side scatter and dead cells were excluded based on propidium iodide staining.
Error bars represent the standard error from three different samples of Forward Scatter Area vs Side Scatter Area (FSC-A vs SSC-A).
Data for 10 cells were collected for each sample and prior to data collection cell debris was excluded by setting a gate on a forward vs side scatter two-dimensional dot plot.
Lymphocytes were gated on forward (FSC) vs side scatter (SSC) plots and B lymphocytes were identified as cells positive for CD19 expression.
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A small plot study involving five rates of urea N (0, 30, 60 90 and 120 kg N ha−1) and two phosphorus fertilizer placement methods (seed-placed vs side-banded) was conducted on the two adjacent fields for the period 2002 2009.
A Forward-scattered light (FSC) vs Side-scattered light (SSC) plot was set up around the bacterial population followed by another region of stained bacterial population in the FL2 vs SSC dot plot.
The forward vs side-scatter parameters were similar for all the cell lines allowing analysis as a single population.
Doublets were excluded using side-scatter height (SSC-H) vs side-scatter width (SSC-W) and forward-scatter height (FSC-H) vs forwardscatter width (FSC-W) parameters.
Dot plots of forward-angle light scatter (FSc) vs side-angle light scatter (SSc) of MCR cells before and after exposure to BCG were analysed using the Flowing v2.4 software (Turku Center for Biotechnology).
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