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Vortex samples, repeat phase separation, pool the aqueous layers and proceed with isopropanol precipitation.
After a brief vortex, samples were centrifuged at 4,000 rpm for 3 minutes.
After 1 min of Vortex, samples were centrifuged at 13,200 RPM [15.7 Relative Centrifugal Force (RCF)] for 12 min.
After a brief vortex, samples were allowed to stand at −20°C for 2 h, and were then centrifuged at 4°C for 30 min.
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Alternately, vortex sample.
pH-values and conductivities of the thoroughly vortexed samples were determined at room temperature by standard electrodes (pH10-Pen, Qcond 2400, both VWR International, Darmstadt, Germany).
Vortexed samples were centrifuged at 4,000g for 30 min at room temperature.
After sonication and vortexing, samples (50 µl) were spiked onto 1.45 cleanclean quartz filter punch and placed in Petri dish.
For the analysis of phage shedding, vortexed samples were also pelleted by centrifugation and supernatants were removed, filtered with 0.2 µM membranes, and titered on ΔSterne to determine PFUs ml−1 of culture.
After vortexing, samples were stored for 12 hours at 4°C and subsequently stained with a 0.2 µm filter sterilized acridine orange (AO) (0.01%w/v) solution and filtered onto a 0.2 µm filter.
After Precellys®24 homogenization or vortexing, samples were centrifuged at 12,000 rpm for 10 min at 4°C.
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