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Vortex briefly.
3. Add 10 μL DEPC H2O to each tube of the template, vortex briefly and centrifuge.
Vortex briefly and then homogenise for 2-3 minutes in a hand held tissue homogenizer to disaggregate cell clumps.
62. Add resuspended DNase Inactivation Reagent, vortexing before use (typically 0.1 volume; 5 μL if no dilution of RNA was made) and mix well (vortex briefly).
Each sample was placed in a vortex briefly to ensure a homogenous mixture, from which 60 μL was aspirated and placed on the bottom of the sample pad.
Add resuspended DNase Inactivation Reagent, vortexing before use (typically 0.1 volume; 5 μL if no dilution of RNA was made) and mix well (vortex briefly).
Similar(54)
The vesicle suspension was vortexed briefly, and tumbled overnight.
For western blotting, protein was crushed with a pestle in a liquid nitrogen-cooled mortar, solubilised in 50 volumes of single-section western blot lysis buffer [16], vortexed, briefly homogenised and sonicated, heated to 94°C for 4 minutes and centrifuged for 3 min at 15 000×g to remove insoluble matter.
The suspension was vortexed briefly and added to cell cultures.
Two ml of cold TCA (25%) were added to the sample which was vortexed briefly.
After vortexing briefly and centrifugation, 5 μl were injected onto the LC-MS/MS system.
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