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"I'm not sure that I'm personally convinced that the volumetric comparison truly captures all that complexity".
Specifically, we designed (a) a fast and robust automated and semi-automated image analysis platform and a quantification tool that can facilitate accurate diagnostic measurements of pulmonary lesions as well as volumetric measurements of anatomical structures, and incorporated (b) an image registration pipeline to our proposed framework for volumetric comparison of serial scans.
Volumetric comparison between groups relied on additional high-resolution T1-weighted images.
The correction of the cortical brain volumes according to head size/total intracranial volume is pertinent for volumetric comparison between subjects and groups, but in our study the comparisons were done for volumes uncorrected for the head size.
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For the volumetric comparisons between the three groups, ANCOVA (GLM) was used, with volume as dependent variable, group as independent variable, and intracranial volume and age as covariates.
Volumetric comparisons between the three groups are summarized in Tables 1, 2. Age and intracranial volume were included as covariates to correct for possible age and cohort effects.
However, our volumetric comparisons have accentuated that the calculation of fetal liver volume essentially overestimates the results obtained by a hydrostatic method.
For volumetric comparisons in Figure 2, one-sided Wilcoxon signed-rank tests were used to identify increases in the volume of BOLD activation between 10 and 40 Hz and 10 and 100 Hz (corrected for multiple comparisons).
Although mean raw volumetric data are presented for transparency purposes and volumetric comparisons across studies (Table 2), all statistical analyses were performed on normalized values following a previously published standard procedure [ 31] and subsequently converted to z-scores (with log transformation when required).
High-resolution structural imaging techniques, combined with quantitative volumetric comparisons of amnesic individuals and matched controls, allow for differentiation among hippocampal subregions and could provide a richer understanding of how the behavioral profiles observed in individuals with hippocampal damage relate to their underlying brain pathology.
In comparison, volumetric registration as done in similar fMRI models would require masking of the superficial skin and cerebral spinal fluid (CSF) layers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com