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Experimental design for five factors (HNO3 and H2O2 volumes, sample weight, microwave power and digestion time) were used for the optimisation of sample digestion.
Although plasma ADM levels have been previously assessed in several small studies of septic patients [ 9, 15- 17], the assays used required large sample volumes, sample extraction, and long incubation times.
Comparisons between sample types within and across domains may also be limited by the different limits of detection for each sample type, which are a result of different sample volumes, sample processes and sample matrix specific attributes, mainly turbidity and PCR inhibitor concentrations.
(B ) Left: pSTAT5 increase over the first 52 hr of culture as a function of IL-2 and IL-2Rα for titrated TRP1 peptide pulsed on C57BL/6 splenocytes (Calibration Series, gray) and tumor samples diluted in equivalent volumes (Sample Series, green).
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The studied variables were drop volume, sample volume, agitation speed, ionic strength, extraction time and ethanol quantity.
The new fiber-based optical microfluidic detectors, designed for nano-volume sample measurement, are presented.
In addition, an on-line concentration method of large volume sample stacking was designed.
Due to the small test cell volume, sample in situ experiments are not foreseen.
Sheet resistance of minimum spray volume sample was too high to be measured by our analyzer.
Vial volume, sample volume/vial volume ratio, pH, adsorption time and temperature have been optimised by means of two experimental designs.
Factors that influenced the IR signals in this method such as the effect of agitation, headspace volume, sample volume, and temperature were examined in this work.
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