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The aim of our study was to investigate HRQL in long-term survivors (longer than 2 years) after ARDS, all of them ventilated with the low-tidal volume protocol.
This allows those performing the study and clinicians reading the results to determine whether the new intervention (for example, the low tidal volume protocol) is superior not only to the high tidal volume protocol, but also current practice.
Roca and coworkers [ 4] studied patients with ALI who were ventilated using the ARDS Network low tidal volume protocol [ 5].
Gas exchange and apoptosis were assessed at the endpoint of five hours using the low-tidal volume protocol.
The modified large volume protocol was selected for further comparison with the performance of the column-based manual method.
We summarized pager call use and outcomes using frequencies of call volume, protocol use, and patient disposition after the Transfer Center encounter.
RNA was extracted independently from three separate trees using the standard-volume protocol (non-LCM) for the RNAqueous-Micro RNA Isolation Kit (Ambion, USA).
If a patient had been weaned from the ventilator but was reintubated for additional mechanical ventilation within 28 days, the same tidal-volume protocol was resumed.
As the modified large volume protocol yielded results comparable to that of the standard large volume protocol, the former was used for further comparison with the external lysis protocol of the "total nucleic acid" kit which uses a starting sample volume of 200 μL.
To confirm that patients in our cohort were ventilated with a low-tidal-volume protocol, we compared the ventilator settings for patients in our study with data from the original ARDS Network trial of lower-tidal-volume ventilation.
In addition, research indicates that reaching temporary fatigue through a low load high-volume protocol results in a similar increase in muscle protein synthesis when compared to a high load protocol with less repetitions [ 64].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com