Sentence examples for volumes of template from inspiring English sources
A recent meta-analysis on NAATs for TB lymphadenitis found that studies which used volumes of template >20 µl were more accurate than studies that used lesser template volumes [17].
After extensive optimization to reduce the volumes of template required and level of background fluorescence, we were unable to detect DNA in any of our extractions.
Reverse transcription (RT) was done in a reaction component of 20 μl, which contained 2 μl miScript Reverse Transcriptase Mix, 2 μl miScript Nucleics Mix, 4 μl miScript HiSpec Buffer, a certain volume of template RNA containing 100 ng total RNA and a little RNase-free water increasing reaction volume to 20 μl.
Volume of template DNA used (2.0 μL; ~50 ng) worked fine for PCR amplification.
The starting population theoretically represented 760,000 E2 and E6 amplicons, and 28,571 HMBS amplicons per 2 μl of NA6 template (the volume of template loaded in each PCR reaction), which equated to a load of 53 HPV16 copies per diploid genome.
A volume-of-interest template [15] as implemented in PMOD (PMOD Technologies Ltd ,Zurich, Switzerland) was used to obtain brain time-activity curves.
In addition, the small volumes of specimen (template) used in each commercial test may offer additional explanations.
The fMRI data of the monkeys were realigned and non-rigidly co-registered with the anatomical volumes of the template brain (M12, same as subject MM1 in [20]) using the Match software [21].
It demonstrates the high surface area (240 m2 g−1) and the pore volume (0.286 ccg−1) of templated silica xerogel (H900) test.
The surface area and pore volume of the template silica xerogel (H900) were observed to be (240 m2 g−1) and (0.286 ccg−1) respectively.
PCR reactions were performed in 10 μL volumes containing 5 μL PerfeCTa™ SYBR green master mix (2×), 0.3 μL each primer (10 μM; 300 nM final concentration), 1 μL volume of DNA template (1 20 ng), and 3.4 μL of PCR-grade water (Solis BioDyne, Tartu, Estonia).
