Because of the large volume of sample required and ethical restrictions, the use of a panel of individual patient sera was not possible or practical.
The sheer volume of samples required setting priorities among biotic groups for processing, leaving some more difficult groups unsorted.
These were outlined by SooHoo and Walker: (1) a large volume of sample is required, (2) separation can take up to 20 min, (3) multiple separations may be required, and (4) continuous processing is difficult [ 20].
The present methodology employs the application of larger acoustic actuator without compromising the volume of sample fluid required.
A future study including a larger volume of samples is required to further determine the role of promoter methylation in the RFC transcription regulation.
These concerns also exist for the process development laboratory, where despite the smaller scale of operations, the volume of experiments (hence cytotoxic samples) required to define a chemical process is often more significant.
On the other hand, salivary analysis using laboratory-based assay methods often requires relatively large volumes of sample and involves multiple steps of sample acquisition, labelling, freezing, transportation, processing in the laboratory (e.g., centrifugation, sorting, aliquoting, and loading into the analyser), analysis, and, finally, results reporting.
EM studies, while very precise in synapse identification, are limited by the intensity of labor required to collect large volumes of sample.
The conductivity titration curves of other samples were similar with that of the ethanol sulfite pretreated sample, but the volumes of alkali required to reach the two inflection points "A" and "B" were different, which implied that the amount of sulfonic group and weak acid groups in these samples was different.
Most methods permit the use of whole-blood, plasma, or serum samples (required volumes are generally ≤200 μL).
