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Because the technology uses large volumes of cold water from the deep ocean, it calls for an extremely long, wide-bore intake pipe.
For DNA precipitation, 10% NaCl and 2 volumes of cold ethanol were added to the solution.
Tissues were immediately homogenized in ten volumes of cold (4 °C) Tris HCl (10 mM, pH 7.4).
The supernatant was precipitated with 1.8 volumes of cold ethanol (99.9% v/v) o/n at 4 °C to remove HA.
Then 2/3rd volumes of cold isopropanol was added and mixed gently and left for 12 h at 4°C to precipitate nucleic acid.
This is also concordant with the new ILCOR guidelines that recently "recommends against prehospital cooling with rapid infusion of large volumes of cold intravenous fluid" [23].
The W-MR sample was homogenized with nine volumes of cold distilled water (2 to 4°C) at a speed of 11,000 rpm for 1 min.
We do not suggest initiating TTM with infusion of large volumes of cold saline solution during transportation to the hospital after CA. (Grade 2−).
To the obtained supernatant, two volumes of cold ethanol were added, stirred till white cotton-like flocs were formed and then left to stand overnight at 4 °C.
The pellets were dissolved in a minimum volume of extracting buffer and dialysed with 50 volumes of cold extracting buffer with three changes overnight.
After centrifugation the supernatant was recovered and precipitated on ice with 4 volumes of cold ethanol 96% v/v, and stored at 4 °C o/n.
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CEO of Professional Science Editing for Scientists @ prosciediting.com