Sentence examples for volumes buffer from inspiring English sources

The products were washed twice with buffer A, twice with buffer A/TI (1 volume buffer A and 3 volumes buffer TI), and one time with buffer TI (25 mmol/L Tris-HCl and 20 mmol/L imidazole [pH 6.8]).

The column was washed with 7 column volumes buffer A and eluted sequentially with 38 %, 46 % and 100buffer B. The resulting fractions containing unbound material (flow through) as well as molecules eluted at each step were collected for further analysis.

Prior to each run, the column was regenerated by flushing with three column volumes buffer B and buffer A, respectively.

Protein partial-specific volumes, buffer viscosities, and buffer densities were calculated using SEDNTERP (Laue et al., 1992).

The column was washed with 6 volumes buffer A before stepwise elution of bound TolA with buffer B (as A, with 500 mM NaCl).

The resin was washed in a column with 10 volumes buffer B (50 mM Tris/HCl, pH 7.8, 500 mM NaCl, 2 mM DTT) and 10 volumes buffer A supplemented with 10 mM imidazol, and eluted with 50 mM Tris/HCl, pH 7.8, 20 mM NaCl, 60 mM imidazol, 2 mM DTT.

The permeate hydrogen is accumulated in a reservoir volume (buffer) connected to the permeate side.

The architecture of Fe3O4 nanorod array offers void space for volume buffer and fluid electrolyte infiltration.

Part of Cu6Sn5 nanoparticles possesses hollow structure, which is also favorable for volume buffer and stress accommodation.

For the enzyme extraction, the rotation velocity, the extraction time, the temperature, and the solvent volume (buffer) were optimized using response surface methodology (RS M.

The Ni-NTA beads were washed with one column volume buffer A containing 50 mM imidazole.