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Free volume was quantified using the positron annihilation lifetime (PAL) technique and gas permeabilities were measured for O2 and H2 as functions of temperature and relative humidity.
The concentration of floral nectar was measured immediately after collection using a temperature compensated refractometer (ATAGO N-10E refractometer, Leo Kübler GmbH, Karlsruhe, Germany) and the nectar volume was quantified using 5 µl micro-capillaries as described in [33].
For each mouse brain the spectrum of the relative amount of metabolites inside the experimental PRESS volume was quantified using the QUEST (quantitation based on quantum estimation) module [124] available inside the Java Magnetic Resonance User Interface (JMRUI) package [125].
ER volume was quantified using ImageJ (NIH freeware, http://rsb.info.nih.gov/nih-image).
The visceral fat volume was quantified using CT imaging [ 17, 18].
The paw volume was quantified using digital plethysmometer before and at 1st, 2nd, 3rd and 4th h after carrageenan administration.
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Trabecular bone of the femoral epiphysis, subchondral cortical bone, and osteophyte volume were quantified using micro-computed tomography (μCT).
BEAS-2B cells were grown in 6 well plates to approximately 80% confluence, trypsinized, and cell diameter and volume were quantified using the Millipore scepter automated cell counter.
Signal intensity was quantified using ImageJ [41].
Spots were identified and volumes were quantified using Progenesis SameSpots software.
Western blot band volumes were quantified using TotalLab 100 software (Nonlinear Dynamics, Newcastle, UK) and normalised against actin.
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